WebBiochemically isolated synaptoneurosome lysates were processed using the EasyPhos platform. Detailed protocols are provided in the associated manuscript. Data Processing Protocol. Mass spectrometry RAW data were processed using MaxQuant and analysed using Perseus. Detailed settings are provided in the associated manuscript. WebSep 24, 2024 · This protocol describes the EasyPhos platform, a high-throughput and user-friendly workflow for reliable identification of phosphopeptides from small amounts (<200 μg) of sample. Sean J. Humphrey ...
Integrative proteomics reveals principles of dynamic …
WebSep 7, 2024 · PXD009227 is an original dataset announced via ProteomeXchange. Dataset Summary Dataset History Publication List Humphrey SJ, Karayel O, James DE, Mann M, … WebSep 6, 2024 · The EasyPhos workflow addresses these and is sufficiently streamlined to enable the analysis of hundreds of phosphoproteomes at a depth of >10,000 quantified phosphorylation sites. Here we... cesma za vodu
High-throughput and high-sensitivity …
WebDec 8, 2024 · In a single run, PhosPiR provides data clean-up, fast data overview, multiple statistical testing, differential expression analysis, phosphosite annotation and translation across species, multilevel enrichment analyses, proteome-wide kinase activity and substrate mapping and network hub analysis. WebThe EasyPhos workflow addresses these and is sufficiently streamlined to enable the analysis of hundreds of phosphoproteomes at a depth of >10,000 quantified … WebHumphrey SJ, Karayel O, James DE, Mann M (2024) High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform. Nat Protoc 13(9):1897–1916 Noya SB, Colameo D, Brüning F, Spinnler A, Mircsof D, Opitz L, Mann M, Tyagarajan SK, Robles MS, Brown SA (2024) The forebrain synaptic transcriptome is organized by clocks but its ... cesme butik otel