NettetThe inteins are internal protein-splicing elements that self-excise themselves from precursor proteins and catalyze ligation of the flanking peptides together [ 12 ]. Based on this special mechanism, many intein-mediated applications were developed in protein refolding [ 13 ], purification [ 14 ], and ligation [ 15 ]. NettetHere we describe a simple, efficient, and potentially low-cost approach-split intein-mediated ultrarapid purification (SIRP)-for both the purification of the desired …

Inteins and affinity resin substitutes for protein …

NettetA novel protein purification method was developed using microbial polyhydroxyalkanoates ( PHA) granule-associated protein phasin, a pH-inducible self-cleaving intein and PHA nanoparticles. Nettet上海展辉生物技术有限公司主营:细胞实验外包,动物实验外包,实验外包公司,western blot,PCR检测,细胞培养,实验外包,动物实验,免疫组化,透射电镜等.上海展辉生物技术有限公司专业提供动物模型定制的技术服务。公司建立的疾病动物模型已达260余种，在国内外疾病动物模型制备种类上名列前茅。 tracy ferro

Self-Cleaving Tags Based on Split Inteins: Increased Reliability ...

NettetInteins are protein segments that auto-catalyze their excision from proteins, offering an alternative to protease cleavage. Used in conjunction with a strong-binding affinity tag, … Nettet1. okt. 2008 · Purification of the fusion protein using chitin affinity chromatography is typically followed by the addition of a small nucleophilic thiol, mercaptoethane sulphonic acid (MESNA). MESNA enables the cleavage of the target protein catalyzed by the intein domain, resulting in the production of a stable C-terminal thioester of the target protein. NettetThe chitin-binding domain (CBD) present in the intein-tag, allows for the affinity purification of the fusion protein using chitin beads. Generally, a column packed with 10 ml of chitin beads (10 ml bed volume or 20 ml chitin beads slurry) should be used for a one liter culture (adjust the amount of beads according to expression level). tracy fetterolf best