Web2 dec. 1974 · Kethoxal forms a stable complex with ribosomal protein in addition to binding to all ribosomal RNA fractions. Approximately 4 30S proteins and 6 50S proteins from 70S reacted-ribosomes bind kethoxal. The reagent, which is specific for guanine residues in RNA, reacts with 23S, 16S and 5S RNA's in 70S ribosomes. WebThis unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension ...
CAS 27762-78-3 Kethoxal - Alfa Chemistry
Web2 mei 2024 · KAS-seq applies N3-kethoxal to label guanines along single-stranded DNA in live cells, enabling characterization of ssDNA-involved transcription dynamics with as little as 1,000 cells. WebMethylene blue dot results are listed as a control. d, Dot blot of isolated total RNA from mES cells that were treated by N3-kethoxal with different periods, 1, 5, 10, 15 and 20 min. e, … bulk cover crop seed
Analysis of kethoxal bound to ribosomal proteins from
WebWe developed N3-kethoxal, an azido derivative of kethoxal that reacts with deoxyguanosine bases of ssDNA in live cells within 5–10 min at 37 °C, allowing the capture of dynamic changes. Downstream biotinylation of labeled DNA occurs via copper-free click chemistry. Altogether, the KAS-seq procedure involves N3-kethoxal labeling, ... WebKethoxal is a novel and potent antiviral agent, modifying guanine containing oligoribonucleotides by reacting selectively with guanine in polynucleotides. These … WebInterest in the chemotherapeutic activity of thiosemicarbazones stems from the discovery of Domagk et al. (1946) that certain monothiosemicarbazones have antitubercular action. Since then, a great many mono- and dithiosemicarbazones have been synthesized and assayed for possible chemotherapeutic activity in a number of systems. crye precision chops