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Peasy-blunt e1 expression vector

WebAug 28, 2024 · The recombinant prokaryotic expression vector pEASY-Blunt E1-IrUGT86A1 with 6×HIS-IrUGT86A1-like fusion protein was obtained with PCR identification, sequencing identification, and transformed into BL21 (DE3) prokaryotic expression competent cells. Protein expression was induced by 0.2 mmol/L IPTG for 16 h in the constant temperature … Web本发明涉及生物技术，具体的是一种乙酰海藻酸钠酯酶及该酯酶在降低铜绿假单胞杆菌frd1耐药性方面的应用。乙酰海藻酸钠酯酶如序列表seq id no:1中碱基所示。所述乙酰海藻酸钠酯酶在降解乙酰化海藻酸钠中的应用。所述乙酰海藻酸钠酯酶在防治铜绿假单胞杆菌感染中的应用 …

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WebpEASY ®-Blunt E1 Expression Kit Products PCR, RT-PCR, qPCR, qRT-PCR Restriction/Modifying Enzymes DNA Molecular Weight Standards Cloning and Mutagenesis System Nucleic Acid Purification Next Generation Sequencing Gene Expression Protein Analysis Protein MW Standards Cell Culture and Detection Exosome Related Serum-free … WebOct 20, 2024 · Briefly, the complete open reading frames (ORFs) of Bip, DNAJB12, and Hsc70 were amplified by RT-PCR and inserted into the pEASY-Blunt E1 expression vector (TransGen Biotech). The resulting plasmids E1-Bip, E1-DNAJB12, and E1-Hsc70 were transformed into Escherichia coli Rosetta for expression by adding isopropyl-β-d … shapewear women plus size postpartum https://leesguysandgals.com

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Web糙皮侧耳新831菌株为河南农业大学生命科学学院应用真菌研究室保藏；pEASY-blunt E1 expression kit、pEASY-blunt cloning kit、TransStart FastPfu DNA polymerase购自北京全式金生物技术有限公司；E.coliDH5α、E.coliBL21（DE3）购自北京博迈德基因技术有限公司；RNA trizol plus购自大连宝 ... WebExpression of the recombinant TrXyn10 The pEASY-Blunt E1-trxyn10 vector was trans-formed into E. coli BL21 (DE3), and the positive clones were isolated for TrXyn10 expression. The transformants were cultured overnight in LB culture medium with 100 lg/ml ampicillin at 37 C and 220 rpm. Then, 1 ml of the cells was added to 100 ml WebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white … shapewear with waist trainer

糙皮侧耳蓝光受体基因Po.WC-1的克隆及分析_参考网

Category:Molecular cloning and prokaryotic expression of RdRp gene of IAPV

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Peasy-blunt e1 expression vector

杜梨PbGID1家族基因克隆、CRISPR/Cas9载体构建及遗传转化

WebpEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. Pricing & Availability Download Catalogue Add to wishlist. View More Details Add to Cart. WebJan 15, 2024 · Among them, the expression of 5-methylthioadenosine/ S -adenosylhomocysteine nucleosidase, which is known to be involved in AI-2 synthesis and cysteine and amino acid metabolism, was upregulated. These findings will lay the foundation to clarify the mechanism of cell-to-cell communication and bacterial …

Peasy-blunt e1 expression vector

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WebpEASY ®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient … WebMay 1, 2024 · Expression analysis of the BoaZDS gene in E. coli The BoaZDS fragment was inserted into the pEASY-Blunt E1 vector, and a recombinant prokaryotic expression vector was constructed. The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells.

Web酿酒酵母(S.cerevisiaeCCIC 1002)由本实验室保藏；宿主菌EscherichiacoliBL21(DE3)为实验室已有资源；E.coliTrans-T1克隆宿主菌和载体pEASY-Blunt E1购自北京全式金(TransGen Biotech)生物技术有限公司. WebJul 22, 2024 · The controls were formed by uninduced and blank strains (NC, negative control, pEASY®-Blunt E1 expression vector), and protein expression was detected using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blot (WB). Following culture at 16°C for 16 h, a large amount of fermentation broth was …

WebNov 19, 2016 · The pEASY-Blunt E1-trxyn10 vector was transformed into E. coli BL21 (DE3), and the positive clones were isolated for TrXyn10 expression. The transformants were cultured overnight in LB culture medium with 100 µg/ml ampicillin at 37 °C and 220 rpm. Then, 1 ml of the cells was added to 100 ml of LB medium at 25 °C and 220 rpm. WebThen msv was cloned into the prokaryotic expression vector pEASY -Blunt E1. The fusion protein msv was expressed by pEASY -Blunt E1 under the induction of isopropyl-β-d …

WebpEASY-Blunt E1 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Sequence Author: TransBionovo (TransGen …

WebFeb 15, 2024 · 表3 表达载体构建体系Tab.3 Expression vector construction system 1.5 杜梨子叶浸染及遗传转化 杜梨子叶再生参照林静[18]的方法，遗传转化参照庞宏光[19]的方法，主要操作包括：杜梨种子消毒、子叶诱导愈伤培养、农杆菌介导的遗传转化、筛选培养基培 … shapeweb.comWebpEASY ®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient … shapewear with zipper on the sideWebOct 15, 2024 · UGT74B1 CDS was ligated with the pEASY-Blunt E1 expression vector and transformed into Trans 1-T1 (TransGene, China), yielding the recombinant vector pEASY -Blunt E1-BoaUGT74B1. The recombinant plasmid and empty plasmid were transferred into E. Coli Transetta (DE3) (TransGene, China). shapewear women\u0027s clothing dressesWebPeasy Blunt E1 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … shapewear women\u0027s molded cup bodysuitWebAug 26, 2024 · Briefly, the recombinant vector (pEASY-Blunt E1-PiGSTd1) was transformed into E. coli BL21 (DE3), and the positive clones were isolated for expression. Cultures were started from single colonies, in LB broth with 50 μg/mL ampicillin in a … shapewear women thighs can use bathroomWebSep 30, 2024 · Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21 (DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach Authors:... shapewebWebpEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no … poodles for adoption california