WebGet $500 voucher for order over USD $5,000 in a year. AKT/PKB Kinase (Human) Assay/Inhibitor Screening Kit is a single-site, non-quantitative immunoassay for AKT activity. Plates are pre-coated with "AKTide-2T" (10), which can be efficiently phosphorylated by AKT1, 2 and 3 on a microtiter plate. The detector antibody is AT-3E2, an antibody that ... WebFeb 28, 2008 · Two protein kinases, JNK1 and p38 alpha (MKK14) (Table 1 ), were incubated on arrays at 30°C for 0, 1, 10, 30, 60 or 120 minute prior to washing and detection. As shown in Figure 4a, signals for each kinase-substrate interaction were greatest on average at 30–60 minutes following addition of the kinase.

TRIM47 activates NF-κB signaling via PKC-ε/PKD3 stabilization …

WebPhospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 37 kinase phosphorylation sites and 2 related total proteins without performing numerous immunoprecipitations … WebThe MAP protein array is used to test the specificity of antibodies to human proteins and to screen for autoantibodies in serum from patients with … haus labs brown eyeliner

Protein kinase substrate identification on functional protein arrays ...

WebAdvantages of membrane-based cytokine arrays and antibody arrays for other targets. Antibody arrays are used to measure many proteins simultaneously in one experiment, … WebProtein kinases are therapeutic targets for many human diseases, but the lack of user-friendly quantitative assays limits the ability to follow the activities of numerous kinases at once (multiplexing). To develop such an assay, we report an array of sulfonamido-oxine (SOX)-labeled peptides showing cross-reactivity to different mitogen-activated protein … WebApr 11, 2024 · each phosphorylated kinase protein. 4. Subtract an averaged background signal from each spot. Use a signal from a clear area of the array or negative control … borderlinx phone number