Sh-sy5y atcc crl-2266
WebNeuroblastoma cell line SH-SY5Y . The SH-SY5Y neuroblastoma cells were obtained from ATCC (#CRL-2266). Cells were cultured in DMEM with 10% FBS and 1X Penicillin-Streptomycin on 0.1% gelatin-coated plates, passaged with 0.05% Trypsin and 0.02% EDTA solution. Cells at passage number 3–10 were used for cytotoxicity assays. WebThe SH-SY5Y neuroblastoma cell line was obtained from ATCC (CRL-2266). The medium was DMEM/F12 (1:1) supplemented with 10% FBS, Glutamax, and 1× P/S. When cells were seeded for neuronal differentiation, 5% FBS was used, and each well of a 24-well plate was coated with 200 µL of a 25 mg mL −1 Laminin–PBS solution.
Sh-sy5y atcc crl-2266
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WebOct 23, 2024 · The SH-SY5Y neuroblastoma cells (Cat#CRL-2266, ATCC, VA, USA) were maintained in complete culture media comprising 88% DMEM supplemented with 4.5 g/L glucose and L-glutamine without sodium pyruvate (Biosera, Nuaille, France); 10% heat-inactivated FBS (Biosera, Nuaille, France), 1% Penicillin-Streptomycin (PS) solution (Gibco, … WebJan 24, 2024 · The most-reported source for access to the SH-SY5Y cell line is the American Type Culture Collection (ATCC, CRL-2266, deposited by JL Biedler). Other sources …
WebDec 4, 2024 · CRL-2266 ™. The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a … WebThe cells can be purchased from ATCC (cat# CRL-2266), ECACC (cat# 94030304) or from other labs, eg. Uppsala University or Stockholm University (Sweden). The native …
WebSH-SY5Y (ATCC ® CRL-2266™), MDA-MB-231 (ATCC ® HTB-26™), and A549 (ATCC ® CCL-185™) cell lines were grown in Dulbecco’s Modified Eagle’s Medium (DMEM)-high glucose (Gibco) supplemented with 10% FBS and 1× … WebCell culture protocol for SH-SY5Y neuroblastoma cells (human, ATCC# CRL-2266) Growth medium: is a 1:1 ratio of ATCC-formulated MEM and F12 • Invitrogen MEM (# 11095-080 …
WebThe cells can be purchased from ATCC (cat# CRL-2266), ECACC (cat# 94030304) or from other labs, eg. Uppsala University or Stockholm University (Sweden). The native Stockholm University cells display a more developed neuronal phenotype as compared to the cells obtained from ECACC. Equipment Fixed Equipment Balance weighing within the 0.0001-1 …
WebDec 31, 2015 · PDF On Jan 1, 2016, Aslı Aybike Doğan and others published COMPARISON OF MICROTISSUE FORMING CAPACITY OF SH-SY5Y AND SK-N-AS CELL LINES ... SH-SY5Y (cat. CRL-2266 TM, ATCC ... tsx564WebJul 5, 2013 · Cells and Viruses. Human neuroblastoma cells SH-SY5Y (ATCC, CRL-2266™) was cultured in Dulbecco's modified Eagle's medium/F-12 (DMEM/F-12, Invitrogen), … tsx55 tacticWebPurchase online ATCC - SH-SY5Y; Neuroblastoma; Human (Homo sapiens). High Quality CRMs, Reference Materials, Proficiency Testing & More at LGC Standards. We use … tsx 550tWebOct 26, 2015 · SH-SY5Y neuroblastoma cells (CRL-2266, ATCC) were maintained in DMEM supplemented with 10 % FBS, 100 Units/mL penicillin and 0.1 mg/mL streptomycin in … pho broth ingredient listhttp://genome.cse.ucsc.edu/ENCODE/protocols/cell/human/SH-SY5Y_Farnham_protocol.pdf tsx550t vs monitorWebApr 10, 2024 · SH-SY5Y cells (a human neuroblastoma cell line; ATCC #CRL-2266) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Nakalai, Japan) containing Ham’s F12 supplemented with 10% fetal bovine serum ... SH-SY5Y/BoDV cells were treated with RA in the presence of the scrambled siRNA (siCont) ... pho brownsville texasWebNov 2, 2024 · Human cell lines SK-N-AS (ATCC CRL-2137), SK-N-DZ (ATCC CRL-2149), SH-SY5Y (ATCC CRL-2266), and A549 (ATCC CCL-185) were purchased from ATCC. Cells were cultured in DMEM/Ham's F-12 50/50 Mix containing l -glutamine and 15 mmol/L HEPES supplemented with 10% (v/v) heat-inactivated FBS and 1% antibiotic and antimycotic … tsx562ist